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Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes.
Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138281pKEW215_AcrIIA13Expression of AcrIIA13 protein
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138282pKEW353_AcrIIA13_truncatedExpression of truncated AcrIIA13 protein
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138283pKEW394_AcrIIA14Expression of AcrIIA14 protein
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138284pKEW395_AcrIIA14_truncatedExpression of truncated AcrIIA14 protein
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138285pKEW397_AcrIIA15Expression of AcrIIA15 protein
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138286pKEW398_AcrIIA15_truncatedExpression of truncated AcrIIA15 protein
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138305pCF525-AcrIIA4_gb39_lentiLenti expression of AcrIIA4
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138306pCF525-AcrIIA5_gb36_lentiLenti expression of AcrIIA5
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138310pCF525-AcrIIA13b_gb76_GF31-2-hom_lentiLenti expression of AcrIIA13b
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138311pCF525-AcrIIA14_gb77_Cand9_lentiLenti expression of AcrIIA14
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138312pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lentiLenti expression of AcrIIA14, C-terminus
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138313pCF525-AcrIIA15_gb79_Cand27_lentiLenti expression of AcrIIA15
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138315pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lentiLenti expression of mTgBFP2
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138316pCF820_U6-sgRNA-EF1a-mCherry2_lentiLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP
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138317pCF823_EFS-Cas9-PuroR_lentiLenti expression of SpyCas9 with Puro selection
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138318pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lentiLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP
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