Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes.
Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
138281pKEW215_AcrIIA13Expression of AcrIIA13 protein
138282pKEW353_AcrIIA13_truncatedExpression of truncated AcrIIA13 protein
138283pKEW394_AcrIIA14Expression of AcrIIA14 protein
138284pKEW395_AcrIIA14_truncatedExpression of truncated AcrIIA14 protein
138285pKEW397_AcrIIA15Expression of AcrIIA15 protein
138286pKEW398_AcrIIA15_truncatedExpression of truncated AcrIIA15 protein
138305pCF525-AcrIIA4_gb39_lentiLenti expression of AcrIIA4
138306pCF525-AcrIIA5_gb36_lentiLenti expression of AcrIIA5
138310pCF525-AcrIIA13b_gb76_GF31-2-hom_lentiLenti expression of AcrIIA13b
138311pCF525-AcrIIA14_gb77_Cand9_lentiLenti expression of AcrIIA14
138312pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lentiLenti expression of AcrIIA14, C-terminus
138313pCF525-AcrIIA15_gb79_Cand27_lentiLenti expression of AcrIIA15
138315pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lentiLenti expression of mTgBFP2
138316pCF820_U6-sgRNA-EF1a-mCherry2_lentiLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP
138317pCF823_EFS-Cas9-PuroR_lentiLenti expression of SpyCas9 with Puro selection
138318pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lentiLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

Antibodies from Article