The chloroalkaloid (-)-acutumine is biosynthesized via a Fe(II)- and 2-oxoglutarate-dependent halogenase in Menispermaceae plants.
Kim CY, Mitchell AJ, Glinkerman CM, Li FS, Pluskal T, Weng JK
Nat Commun. 2020 Apr 20;11(1):1867. doi: 10.1038/s41467-020-15777-w. PubMed Article
Kim CY, Mitchell AJ, Glinkerman CM, Li FS, Pluskal T, Weng JK
Nat Commun. 2020 Apr 20;11(1):1867. doi: 10.1038/s41467-020-15777-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140128 | pHis8-4b-SaDAH | Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli |
| 140129 | pHis8-4b-McDAH | Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli |
| 140130 | pHIs8-4b-SaDAH::G226D | Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli |