Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel Trafficking.
Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 10.1016/j.celrep.2019.08.079. PubMed Article
Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 10.1016/j.celrep.2019.08.079. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206066 | Cav2.2 HA EIVK (E1658K) pcDNA3 | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface |
| 206069 | Cav2.1 HA pcDNA3 | expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II |
| 206081 | Cav2.2 HA EIA (E314A) pCAGGS | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface |
| 206082 | Cav2.2 HA EIVA (E1658A) pCAGGS | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface |
| 206093 | GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3 | Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface |
| 206096 | Cav2.2 HA EIVK (E1658K) pCAGGS | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface |
| 206098 | Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface |
| 206114 | Cav2.2 HA E1658A (EIVA) pcDNA3 | expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface |
| 206115 | Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS | expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface |
| 206120 | GFP Cav2.2 HA D122A pcDNA3 | expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1 |
| 206121 | Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3 | expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding |
| 206124 | Cav2.1 HA EIA (E320A) pcDNA3 | expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding |
| 206125 | Cav2.1 EIVA (E1707A) pcDNA3 | expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding |