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Nanobody production can be simplified by direct secretion from Escherichia coli.
Iwaki T, Hara K, Umemura K
Protein Expr Purif. 2020 Feb 13;170:105607. doi: 10.1016/j.pep.2020.105607.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140699pMAK461NBαRb-IgG-Fc
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140700pMAK462NBαMs/Rt-IgG1-Fc
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140701pMAK463NBαMs-IgK
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140702pMAK464NBαEGFP
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Antibodies from Article