|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140699||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer caatttcacacaggaaacagccag
- 3′ sequencing primer gtcctactcaggagagcgttc (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMAK461 was a gift from Takayuki Iwaki (Addgene plasmid # 140699 ; http://n2t.net/addgene:140699 ; RRID:Addgene_140699)
For your References section:Nanobody production can be simplified by direct secretion from Escherichia coli. Iwaki T, Hara K, Umemura K. Protein Expr Purif. 2020 Feb 13;170:105607. doi: 10.1016/j.pep.2020.105607. 10.1016/j.pep.2020.105607 PubMed 32062022