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Engineering an enhanced voltage-sensing phosphatase.
Kawanabe A, Mizutani N, Polat OK, Yonezawa T, Kawai T, Mori MX, Okamura Y
J Gen Physiol. 2020 May 4;152(5). pii: 133870. doi: 10.1085/jgp.201912491. PubMed Article

Plasmids from Article

ID Plasmid Purpose
140892CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.
140893CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

Antibodies from Article