CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
(Plasmid #140892)

• Purpose: Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.
• Depositing Lab: Yasushi Okamura
• Publication: Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 133870. doi: 10.1085/jgp.201912491.

Backbone

• Vector backbone: pcDNA3.1(-)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CiDr-VSP L223F-mCherry
• Species: D. rerio (zebrafish); Ciona intestinalis
• Insert Size (bp): 2433
• Mutation: changed 223L to F in Dr-VSP (zebrafish TPTE)
• GenBank ID: NM_001025458.1 NM_001033826.1
• Entrez Gene: tpte (a.k.a. tpip, vsp, wu:fd20e11, wu:fi24b06)
• Entrez Gene: vsp
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry