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Unblending of Transcriptional Condensates in Human Repeat Expansion Disease.
Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S, Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, Ibrahim DM, Hnisz D
Cell. 2020 May 28;181(5):1062-1079.e30. doi: 10.1016/j.cell.2020.04.018. Epub 2020 May 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
145228pGAL4-DBD-BHLHE41-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR
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145229pGAL4-DBD-BHLHE41wt-IDRExpresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR
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145230pGAL4-DBD-EOMES-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR
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145231pGAL4-DBD-EOMESwt-IDRExpresses fusion of GAL4 DNA-binding domain and EOMESwt IDR
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145232pGAL4-DBD-GSX2-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR
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145233pGAL4-DBD-GSX2wt-IDRExpresses fusion of GAL4 DNA-binding domain and GSX2wt IDR
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145234pGAL4-DBD-HEY2-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR
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145235pGAL4-DBD-HEY2wt-IDRExpresses fusion of GAL4 DNA-binding domain and HEY2wt IDR
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145236pGAL4-DBD-HOXA13-15A-IDRExpresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR
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145237pGAL4-DBD-HOXA13-44A-IDRExpresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR
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145238pGAL4-DBD-HOXA13-58A-IDRExpresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR
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145239pGAL4-DBD-HOXA13+7A-IDRExpresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR
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145240pGAL4-DBD-HOXA13wt-IDRExpresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR
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145241pGAL4-DBD-HOXD11-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR
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145242pGAL4-DBD-HOXD11wt-IDRExpresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR
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145243pGAL4-DBD-HOXD13-Adel-15A-IDRExpresses fusion of GAL4 DNA-binding domain and HOXD13-Adel-15A IDR
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145244pGAL4-DBD-HOXD13wt-IDRExpresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR
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145245pGAL4-DBD-MCSBackbone vector expressing GAL4 DNA-binding domain followed by MCS
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145246pGAL4-DBD-MCS-longBackbone vector expressing GAL4 DNA-binding domain followed by MCS
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145247pGAL4-DBD-MNX1-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and MNX1-Adel IDR
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145248pGAL4-DBD-MNX1wt-IDRExpresses fusion of GAL4 DNA-binding domain and MNX1wt IDR
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145249pGAL4-DBD-OLIG2-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR
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145250pGAL4-DBD-OLIG2wt-IDRExpresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR
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145251pGAL4-DBD-RUNX2+10A-IDRExpresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR
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145252pGAL4-DBD-RUNX2wt-IDRExpresses fusion of GAL4 DNA-binding domain and RUNX2Awt IDR
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145253pGAL4-DBD-TBX1-Adel-IDRExpresses fusion of GAL4 DNA-binding domain and TBX1-Adel IDR
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145254pGAL4-DBD-TBX1wt-IDRExpresses fusion of GAL4 DNA-binding domain and TBX1wt IDR
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145255pCFP-LacI-HOXD13wt-IDRExpresses fusion of ECFP-LacI and HOXD13wt IDR
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145256pCFP-LacI-HOXD13(+7A)-IDRExpresses fusion of ECFP-LacI and HOXD13+7A IDR
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145257pCFP-LacI-HOXD13(+10A)-IDRExpresses fusion of ECFP-LacI and HOXD13+10A IDR
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145258pHR-HOXD13-IDR-mCherry-CRY2Mammalian Expression of Opto HOXD13 IDR
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145259pHR-mCherry-CRY2-NLSMammalian Expression of Nuclear Control OptoConstruct with SV40 NLS
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145260pHR-HOXD13-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS
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145261pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS
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145262pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS
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145263pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS
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145265pHR-HOXA13-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS
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145266pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS
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145267pHR-RUNX2-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS
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145268pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS
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145269pHR-TBP(38Q)-IDR-mCherry-Cry2-NLSMammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS
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145270pHR-TBP(53Q)-IDR-mCherry-Cry2-NLSMammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS
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145271pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS
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145272pHR-HOXD13(-15A)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13(-15A) IDR with SV40 NLS
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145273pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLSMammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLS
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145274pHR-MCS-YFP-NLSMammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS
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145275pHR-MED1trunc-IDR-YFP-NLSMammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS
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145276pHR-MCS-MED1long-IDR-YFP-NLSMammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS
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145277pHR-HOXD13-IDR-YFP-NLSMammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS
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145278pHR-HOXD13(+7A)-IDR-YFP-NLSMammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS
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145279pET-45b-mCherryBacterial Expression of 6xHis mCherry Fusion Protein
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145280pET-45b-HOXD13-IDR-mCherryBacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein
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145281pET-45b-HOXD13(+7A)-IDR-mCherryBacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein
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145282pET-45b-HOXD13(+10A)-IDR-mCherryBacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein
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145283pET-45b-TBP(38Q)-IDR-mCherryBacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein
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145284pET-45b-HOXA13(WT)-IDR-mCherryBacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein
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145285pET-45b-HOXA13(+7A)-IDR-mCherryBacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein
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145286pET-45b-RUNX2(WT)-IDR-mCherryBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein
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145287pET-45b-RUNX2(+10A)-IDR-mCherryBacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein
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145288RCASBP(A)-3xFlag-mHoxd13wtAvian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag
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145289RCASBP(A)-3xFlag-mHoxd13+7AlaAvian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag
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