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A robust CRISPR-Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair.
Eki R, She J, Parlak M, Benamar M, Du KP, Kumar P, Abbas T
Nucleic Acids Res. 2020 Oct 17. pii: 5929239. doi: 10.1093/nar/gkaa897.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
149355lenti-CDDRCRISPR/Cas9-based dual-fluorescent DSB repair reporter
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149369Cas9-sgRNA-AExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
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149370Cas9-sgRNA-A+BExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
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149372BFP Homology DonorPromoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection
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