A robust CRISPR-Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair.
Eki R, She J, Parlak M, Benamar M, Du KP, Kumar P, Abbas T
Nucleic Acids Res. 2020 Oct 17. pii: 5929239. doi: 10.1093/nar/gkaa897. PubMed Article
Eki R, She J, Parlak M, Benamar M, Du KP, Kumar P, Abbas T
Nucleic Acids Res. 2020 Oct 17. pii: 5929239. doi: 10.1093/nar/gkaa897. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149355 | lenti-CDDR | CRISPR/Cas9-based dual-fluorescent DSB repair reporter |
| 149369 | Cas9-sgRNA-A | Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter |
| 149370 | Cas9-sgRNA-A+B | Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites |
| 149372 | BFP Homology Donor | Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection |