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Creation of Golden Gate constructs for gene doctoring.
Thomson NM, Zhang C, Trampari E, Pallen MJ
BMC Biotechnol. 2020 Oct 7;20(1):54. doi: 10.1186/s12896-020-00648-5. PubMed Article

Plasmids from Article

ID Plasmid Purpose
149377pDOC-GGChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
149379mUAVbla:tetR 5′mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

Antibodies from Article