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(Plasmid #149377)


Item Catalog # Description Quantity Price (USD)
Plasmid 149377 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3378
  • Total vector size (bp) 5960
  • Modifications to backbone
    Vector backbone fragment containing sacB gene generated by PCR with forward primer GGTAGTGTGGCGAGAGTAGGGAACTGCCAG and reverse primer CCAATTCTGACACATTTCCCCGAAAAGTGCC
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Fragment containing a kanamycin resistance cassette
  • Alt name
  • Alt name
  • Alt name
  • Species
    Synthetic; E. coli
  • Insert Size (bp)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
  • Species
    Synthetic; E. coli
  • Insert Size (bp)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
  • Species
    Synthetic; E. coli
  • Insert Size (bp)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Fragment containing an I-SceI recognition sequence
  • Species
  • Insert Size (bp)

Cloning Information for Gene/Insert 4

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pDOC-K received from Stephen Busby (University of Birmingham, UK)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDOC-GG was a gift from Mark Pallen (Addgene plasmid # 149377 ; ; RRID:Addgene_149377)
  • For your References section:

    Creation of Golden Gate constructs for gene doctoring. Thomson NM, Zhang C, Trampari E, Pallen MJ. BMC Biotechnol. 2020 Oct 7;20(1):54. doi: 10.1186/s12896-020-00648-5. 10.1186/s12896-020-00648-5 PubMed 33028286