Optogenetic control of gene expression in plants in the presence of ambient white light.
Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, Simon R, Zurbriggen MD
Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y. Epub 2020 Jun 29. PubMed Article
Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, Simon R, Zurbriggen MD
Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y. Epub 2020 Jun 29. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155321 | pMZ827 | Constitutive expression of E-PIF6(1-100) in plants |
| 155322 | pMZ828 | Constitutive expression of PhyB(1-650)-VP16 in plants |
| 155323 | pNBA001 | Binary vector for expression of PULSE-controlled EFR-GFP |
| 155324 | pNBA002 | Binary vector for expression of PULSE-controlled GBP-mCherry |
| 155325 | pROF018 | Constitutive expression of KRAB-EL222 in plants |
| 155326 | pROF021 | Expression of PULSE-controlled FLuc |
| 155327 | pROF050 | Constitutive expression of BRD-EL222 in plants |
| 155328 | pROF051 | Constitutive expression of SRDX-EL222 in plants |
| 155329 | pROF141 | Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter |
| 155330 | pROF148 | Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. |
| 155331 | pROF346 | Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette. |
| 155332 | pROF366 | Expression of FLuc under the control of AtAP1 promoter |
| 155333 | pROF394 | Expression of PULSE-controlled LFY-VP16-2A-RLuc |
| 155334 | pROF402 | Binary vector for expression of FLuc controlled by the blue light-repressible system |
| 155335 | pROF417 | Constitutive expression LFY-VP16-2A-RLuc in plants |
| 155336 | pROF441 | Binary vector for expression of a gRNA targeting AtAP1 promoter |
| 155338 | BM00369 | Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette. |
| 155339 | BM00654 | Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette. |