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CRISPRi-mediated Programming Essential Gene can as a Direct Enzymatic Performance Evaluation & Determination (DEPEND) System.
Tan SI, Yu PJ, Ng IS
Biotechnol Bioeng. 2020 May 27. doi: 10.1002/bit.27443.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
154306pSUI-SynP-sfGFPExpression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescence
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154307pSM-dCas9::ApShuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.
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154341pSUI-SynP-CAS1sgExpression of the sgRNA targeting to the E. coli can gene
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154342pSUI-SynP-CAS2sgExpression of the sgRNA targeting to the E. coli can gene
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154343pSUI-SynP-CANTsgExpression of the sgRNA targeting to the E. coli can gene
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154344pSUI-SynP-CAPsgExpression of the sgRNA targeting to the E. coli can gene
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