Multi-copy targeted integration for accelerated development of high-producing CHO cells.
Sergeeva D, Lee GM, Nielsen LK, Grav LM
ACS Synth Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322. PubMed Article
Sergeeva D, Lee GM, Nielsen LK, Grav LM
ACS Synth Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154826 | pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR | Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A) |
| 154827 | pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR | Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9) |
| 154828 | pJ204-loxP-100RPU.2-coEPO-lox2272 | Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin |
| 154829 | pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272 | Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin |
| 154830 | pJ607-CMV-GFP-2A-Cas9 | GFP-2A-Cas9 (CHO codon-optimized) |
| 154831 | pRSFDuet1-sgRNA(siteA) | sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells |
| 154832 | pRSFDuet1-sgRNA(siteT9) | sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells |
| 154833 | pJ204-loxP-100RPU.2-ETN-lox2272 | Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept |
| 154834 | pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272 | Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept |