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CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
Moreb EA, Hutmacher M, Lynch MD
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
154919pKDsgRNA-pgaCaTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes
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154920pKDsgRNA-pheTaTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes
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154921pKDsgRNA-mqoaTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes
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154922pKDsgRNA-hisFaTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes
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154923pKDsgRNA-ampGaTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes
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154924pEM-Cas9HF1-D1135EaTc inducible Cas9HF1 with D1135E mutation
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154925pEM-eCas9aTc inducible eCas9
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154926pEM-eCas9-D1135EaTc inducible eCas9 with D1135E mutation
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154927pEM-OptiCas9aTc inducible OptiCas9
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154928pEM-OptiCas9-D1135EaTc inducible OptiCas9 with D1135E mutation
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154929pEM-HypaCas9aTc inducible HypaCas9
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154930pEM-HypaCas9-D1135EaTc inducible HypaCas9 with D1135E mutation
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154931pEM-Cas9-D1135EaTc inducible Cas9 with D1135E mutation
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154932pNT-1515 designed non-target sites
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154933pNT-1212 designed non-target sites
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154934pNT-33 designed non-target sites
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154935pNT-11 designed non-target site
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