Large-scale tethered function assays identify factors that regulate mRNA stability and translation.
Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE, Aigner S, Yeo GW
Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 10.1038/s41594-020-0477-6. Epub 2020 Aug 17. PubMed Article
Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE, Aigner S, Yeo GW
Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 10.1038/s41594-020-0477-6. Epub 2020 Aug 17. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155396 | ADARB1 | For use in RBP tethering screen |
| 155397 | ADD1 | For use in RBP tethering screen |
| 155402 | ALDH18A1 | For use in RBP tethering screen |
| 155425 | BTG1 | For use in RBP tethering screen |
| 155427 | BTG3 | For use in RBP tethering screen |
| 155428 | BTG4 | For use in RBP tethering screen |
| 155440 | CCNL1 | For use in RBP tethering screen |
| 155441 | CCNL2 | For use in RBP tethering screen |
| 155444 | CDC40 | For use in RBP tethering screen |
| 155452 | CIR1 | For use in RBP tethering screen |
| 155458 | CLK1 | For use in RBP tethering screen |
| 155459 | CLK2 | For use in RBP tethering screen |
| 155462 | CLP1 | For use in RBP tethering screen |
| 155466 | CNOT2 | For use in RBP tethering screen |
| 155467 | CNOT3 | For use in RBP tethering screen |
| 155472 | CPEB1 | For use in RBP tethering screen |
| 155475 | CPSF1 | For use in RBP tethering screen |
| 155477 | CPSF4 | For use in RBP tethering screen |
| 155478 | CPSF4-1 | For use in RBP tethering screen |
| 155479 | CPSF4L | For use in RBP tethering screen |
| 155480 | CPSF5 | For use in RBP tethering screen |
| 155483 | CRABP2 | For use in RBP tethering screen |
| 155484 | CRKRS | For use in RBP tethering screen |
| 155485 | CSTF1 | For use in RBP tethering screen |
| 155487 | CSTF3 | For use in RBP tethering screen |
| 155498 | DCP1A | For use in RBP tethering screen |
| 155499 | DCP2 | For use in RBP tethering screen |
| 155502 | DDX11 | For use in RBP tethering screen |
| 155503 | DDX11-1 | For use in RBP tethering screen |
| 155510 | DDX21 | For use in RBP tethering screen |
| 155512 | DDX24 | For use in RBP tethering screen |
| 155513 | DDX27 | For use in RBP tethering screen |
| 155514 | DDX27-1 | For use in RBP tethering screen |
| 155523 | DDX5 | For use in RBP tethering screen |
| 155530 | DDX56 | For use in RBP tethering screen |
| 155538 | DHX35 | For use in RBP tethering screen |
| 155539 | DHX37 | For use in RBP tethering screen |
| 155540 | DHX40 | For use in RBP tethering screen |
| 155542 | DHX58 | For use in RBP tethering screen |
| 155549 | DMGDH | For use in RBP tethering screen |
| 155550 | DMPK | For use in RBP tethering screen |
| 155561 | EDC3 | For use in RBP tethering screen |
| 155584 | ELMOD3 | For use in RBP tethering screen |
| 155589 | EXOSC10 | For use in RBP tethering screen |
| 155590 | EXOSC2 | For use in RBP tethering screen |
| 155605 | FIP1L1 | For use in RBP tethering screen |
| 155613 | FUBP1 | For use in RBP tethering screen |
| 155618 | FZD8 | For use in RBP tethering screen |
| 155622 | GAPDH | For use in RBP tethering screen |
| 155625 | GLE1 | For use in RBP tethering screen |
| 155670 | HSPB1 | For use in RBP tethering screen |
| 155676 | IGF2BP1 | For use in RBP tethering screen |
| 155681 | INTS6 | For use in RBP tethering screen |
| 155687 | KIF1C | For use in RBP tethering screen |
| 155713 | LUC7L | For use in RBP tethering screen |
| 155725 | METTL3 | For use in RBP tethering screen |
| 155763 | NAP1L3 | For use in RBP tethering screen |
| 155793 | NSUN2 | For use in RBP tethering screen |
| 155794 | NUDT16 | For use in RBP tethering screen |
| 155795 | NUDT16L1 | For use in RBP tethering screen |
| 155805 | PABPC1 | For use in RBP tethering screen |
| 155807 | PABPN1L | For use in RBP tethering screen |
| 155821 | PHF6 | For use in RBP tethering screen |
| 155827 | PLRG1 | For use in RBP tethering screen |
| 155830 | PNRC2 | For use in RBP tethering screen |
| 155836 | PPAPDC2 | For use in RBP tethering screen |
| 155838 | PPIB | For use in RBP tethering screen |
| 155843 | PPP1CA | For use in RBP tethering screen |
| 155846 | PRPF18 | For use in RBP tethering screen |
| 155847 | PRPF19 | For use in RBP tethering screen |
| 155848 | PRPF3 | For use in RBP tethering screen |
| 155850 | PRPF4 | For use in RBP tethering screen |
| 155851 | PRPF40A | For use in RBP tethering screen |
| 155853 | PRPF8 | For use in RBP tethering screen |
| 155877 | RALYL-1 | For use in RBP tethering screen |
| 155879 | RBBP6 | For use in RBP tethering screen |
| 155889 | RBM22 | For use in RBP tethering screen |
| 155899 | RBM38 | For use in RBP tethering screen |
| 155902 | RBM41 | For use in RBP tethering screen |
| 155910 | RBM8A | For use in RBP tethering screen |
| 155915 | RBMX2-1 | For use in RBP tethering screen |
| 155983 | RSRC1 | For use in RBP tethering screen |
| 155986 | RY1 | For use in RBP tethering screen |
| 155999 | SERPINH1 | For use in RBP tethering screen |
| 156001 | SF3A2 | For use in RBP tethering screen |
| 156003 | SF3B3 | For use in RBP tethering screen |
| 156004 | SF3B3-1 | For use in RBP tethering screen |
| 156007 | SFRS10 | For use in RBP tethering screen |
| 156009 | SFRS16 | For use in RBP tethering screen |
| 156010 | SFRS17A | For use in RBP tethering screen |
| 156011 | SFRS17A-1 | For use in RBP tethering screen |
| 156012 | SFRS2IP | For use in RBP tethering screen |
| 156014 | SKIV2L | For use in RBP tethering screen |
| 156020 | SMG6 | For use in RBP tethering screen |
| 156022 | SMN1-1 | For use in RBP tethering screen |
| 156034 | SNW1 | For use in RBP tethering screen |
| 156037 | SR140 | For use in RBP tethering screen |
| 156071 | SYMPK | For use in RBP tethering screen |
| 156073 | TARDBP | For use in RBP tethering screen |
| 156078 | TERT | For use in RBP tethering screen |
| 156081 | THOC1 | For use in RBP tethering screen |
| 156082 | THOC3 | For use in RBP tethering screen |
| 156083 | THOC4 | For use in RBP tethering screen |
| 156084 | THOC5 | For use in RBP tethering screen |
| 156085 | THOC6 | For use in RBP tethering screen |
| 156089 | TOB1 | For use in RBP tethering screen |
| 156090 | TOB2 | For use in RBP tethering screen |
| 156091 | TOE1 | For use in RBP tethering screen |
| 156140 | ZCCHC6 | For use in RBP tethering screen |
| 156149 | ZFP36L2 | For use in RBP tethering screen |
| 156152 | ZNF9 | For use in RBP tethering screen |
| 156158 | PRPF4-1 | For use in RBP tethering screen |
| 198351 | pTET2-lucCP-3MS2 | Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR |
| 198352 | pTET2-RlucCP | Part of Firefly reporter system, encodes Renilla luciferase |
| 198353 | pTET2-lucCP | Part of Renilla reporter system, encodes Firefly luciferase |
| 198354 | pTET2-RlucCP-3MS2 | Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR |