Donor plasmids for phenotypically neutral chromosomal gene insertions in Enterobacteriaceae.
Holden ER, Wickham GJ, Webber MA, Thomson NM, Trampari E
Microbiology (Reading). 2020 Nov 23. doi: 10.1099/mic.0.000994. PubMed Article
Holden ER, Wickham GJ, Webber MA, Thomson NM, Trampari E
Microbiology (Reading). 2020 Nov 23. doi: 10.1099/mic.0.000994. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158058 | pDOC-K-glmS | Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker |
| 158059 | pDOC-K-glmS-GFPfor | Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker |
| 158060 | pDOC-K-glmS-GFPrev | Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker |
| 158061 | pDOC-K-glmS-lacIZ | Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome |
| 158062 | pDOC-K-glmS-mPlum | Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker |