A rationally enhanced red fluorescent protein expands the utility of FRET biosensors.
Mo GCH, Posner C, Rodriguez EA, Sun T, Zhang J
Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x. PubMed Article
Mo GCH, Posner C, Rodriguez EA, Sun T, Zhang J
Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173014 | pcDNA3-StagRFP | Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression. |
| 173015 | pcDNA3-GR-AKAR3 | Improved green-red FRET-based PKA activity reporter. |
| 173016 | pcDNA3-Lyn-GR-AKAR3 | Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter. |
| 173017 | pcDNA3-GR-ICUE2 | Improved green-red FRET based cAMP indicator. |
| 173018 | pcDNA3-Tsapphire-StagRFP-SrcAR | Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications. |
| 173019 | pcDNA3-smURFP-StagRFP-EKAR-EV | Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications. |
| 173020 | pcDNA3-Venus-StagRFP-AktAR | Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications. |