CRISPR–Act3.0 for highly efficient multiplexed gene activation in plants
Pan , Changtian, Wu, Xincheng, Markel, Kasey, Malzahn, Aimee A., Kundagrami, Neil, Sretenovic, Simon, Zhang, Yingxiao, Cheng, Yanhao, Shih, Patrick M. & Qi , Yiping
Nat. Plants (2021) Article
Pan , Changtian, Wu, Xincheng, Markel, Kasey, Malzahn, Aimee A., Kundagrami, Neil, Sretenovic, Simon, Zhang, Yingxiao, Cheng, Yanhao, Shih, Patrick M. & Qi , Yiping
Nat. Plants (2021) Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 158393 | pYPQ131-tRNA2.0 | Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
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| 158394 | pYPQ132-tRNA2.0 | Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
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| 158395 | pYPQ133-tRNA2.0 | Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
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| 158396 | pYPQ134-tRNA2.0 | Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
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| 158397 | pYPQ135-tRNA2.0 | Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
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| 158398 | pYPQ136-tRNA2.0 | Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter |
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| 158400 | pYPQ143-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAs |
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| 158402 | pYPQ144-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs |
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| 158403 | pYPQ145-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs |
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| 158404 | pYPQ146-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs |
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| 158406 | pYPQ141-ZmUbi-RZ-Aac.4 | Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter |
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| 158407 | pYPQ141-ZmUbi-RZ-Aa3.8.5 | Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter |
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| 158408 | pYPQ-dpcoCas9-Act3.0 | CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
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| 158409 | pYPQ-dpcoCas9-TV | CRISPR-dpcoCas9 fused with TV activator for transcriptional activation |
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| 158410 | pYPQ-dpcoCas9-SunTag | CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation |
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| 158411 | pYPQ-dpcoCas9-EV2.1 | CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation |
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| 158413 | pYPQ-dAaCas12b-Act3.0 | CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
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| 158414 | pYPQ-dzCas9-Act3.0 | CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
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| 158415 | pYPQ-dzCas9-NG-Act3.0 | CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
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| 158416 | pYPQ-dSpRY-Act3.0 | CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator. |
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| 158578 | pYPQ142-ZmUbi-tRNA | Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs |
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| 167158 | pYPQ134B2.0 | Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
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| 167159 | pYPQ135B2.0 | Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
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| 167160 | pYPQ136B2.0 | Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
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| 167161 | pYPQ141D-gRNA2.1 | Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter |
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