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A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.
Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
158713pNHEJ-Cas12aCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum
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158714pNHEJ-Cas12a-recXCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX
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158715pNHEJ-Cas9-recXCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX
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158717pNHEJ-recAmu-sacBCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.
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158718pNHEJ-recX-sacBCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.
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158719pYC1640CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
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158720pYC1446Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA
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158721pYC2085CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
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158722pYC1876Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA
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