PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||158714||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4682
- Promoter Pmyc
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer - (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNHEJ-Cas12a-recX was a gift from Yi-Cheng Sun (Addgene plasmid # 158714 ; http://n2t.net/addgene:158714 ; RRID:Addgene_158714)
For your References section:A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC. mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19. 10.1128/mBio.02364-19 PubMed 31992616