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Genetically encoded live-cell sensor for tyrosinated microtubules.
Kesarwani S, Lama P, Chandra A, Reddy PP, Jijumon AS, Bodakuntla S, Rao BM, Janke C, Das R, Sirajuddin M
J Cell Biol. 2020 Oct 5;219(10). pii: 152071. doi: 10.1083/jcb.201912107. PubMed Article

Plasmids from Article

ID Plasmid Purpose
158751TagRFP-T_A1aY1Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.
158752TagBFP_A1aY1Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.
1587536x-His_A1aY1_GFPBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification
1587546xHis-TagRFP-T_A1aY1Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Antibodies from Article