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Structural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7.
Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
Science. 2022 Jan 21;375(6578):326-331. doi: 10.1126/science.abf6154. Epub 2022 Jan 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159717pOmnibac_ZZ_linker_ybbR_TauExpresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag
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159718pOmnibac_ZZ_linker_ybbR_MAP7Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag
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159719pOmnibac_ZZ_linker_ybbR_Tau MTBDExpresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tag
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159720pOmnibac_ZZ_linker_K560_GFP_SNAPExpresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag
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159721pOmnibac_ZZ_linker_K490_GFP_SNAPExpresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag
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159727pOmnibac_ZZ_linker_K963_GFP_SNAPExpresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag
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159728pOmnibac_ZZ_linker_ybbR_MAP7_NExpresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tag
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Antibodies from Article