pOmnibac_ZZ_linker_K560_GFP_SNAP
(Plasmid #159720)

• Purpose: Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag
• Depositing Lab: Ahmet Yildiz
• Publication: Ferro et al Science. 2022 Jan 21;375(6578):326-331. doi: 10.1126/science.abf6154. Epub 2022 Jan 20.

Backbone

• Vector backbone: pOmnibac
• Total vector size (bp): 8016
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KIF5B (only amino acids 1-560), K560
• Alt name: Kinesin-1 heavy chain
• Alt name: Isoform P33176-1
• Alt name: UniProtKB - P33176 (KINH_HUMAN)
• Species: H. sapiens (human)
• Insert Size (bp): 1680
• Mutation: Only amino acids 1-560
• GenBank ID: 3799
• Entrez Gene: KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
• Tags / Fusion Proteins:
  • ZZ affinity tag (N terminal on insert)
  • GFP and SNAP tags (C terminal on insert)

Resource Information

• Supplemental Documents:
  • K560 pOmnibac Brooks.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry