CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks.
Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA
Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143. PubMed Article
Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA
Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171666 | pJCC_039 FnCas12a | For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag |
| 171667 | pJCC_058 FnCas12a D917A | For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag |
| 171668 | pJCC_075 AsCas12a D908A | For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag |
| 171669 | pJCC_076 AsCas12a R1226A | For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag |
| 171670 | pJCC_083 Cas12i1 | For bacterial expression of Cas12i1 with an N-terminal His-MBP tag |
| 171671 | pJCC_084 Cas12i1 D647A | For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag |