pJCC_058 FnCas12a D917A
(Plasmid #171667)

• Purpose: For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag
• Depositing Lab: Jennifer Doudna
• Publication: Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143.

Backbone

• Vector backbone: p2CT
• Backbone manufacturer: UC Berkeley MacroLab
• Backbone size w/o insert (bp): 4717
• Total vector size (bp): 8662
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FnCas12a D917A
• Alt name: FnCpf1 D917A
• Species: Francisella novicida
• Insert Size (bp): 3945
• Mutation: changed aspartate 917 to alanine
• Tags / Fusion Proteins:
  • sortase A sorting motif (C terminal on insert)
  • 6X His (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pJCC_058 FnCas12a D917A
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 113432 (pMBP-FnCas12a)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry