Synthetic protein-binding DNA sponge as a tool to tune gene expression and mitigate protein toxicity.
Wan X, Pinto F, Yu L, Wang B
Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9. PubMed Article
Wan X, Pinto F, Yu L, Wang B
Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160818 | pXW117Ptet2-gfp | aTc-responsive single-layered circuit |
| 160819 | pXW101Plux2-gfp | AHL-responsive single-layered circuit |
| 160820 | pXW101Plux2-33Ptet2-gfp | Two-layered AHL-responsive circuit containing a TetR-based inverter |
| 160821 | pXW101Plux2-32Ptet2-gfp | Two-layered AHL-responsive circuit containing a TetR-based inverter |
| 160822 | pXW109PmerT-Pecf11-gfp | Two-layered mercury responsive circuit carrying an ECF11-based amplifier |
| 160823 | pXW101Plux2-Pecf11-gfp | Two-layered AHL-responsive circuit carrying an ECF11-based amplifier |
| 160824 | pXWStetO | 1 repeat of tetO sponge |
| 160825 | pXWS10tetO | 10 repeats of tetO sponge |
| 160826 | pXWS20tetO | 20 repeats of tetO sponge |
| 160827 | pXWS40tetO | 40 repeats of tetO sponge |
| 160828 | pXWS80tetO | 80 repeat of tetO sponge |
| 160829 | pXWS160tetO | 160 repeats of tetO sponge |
| 160830 | pXWS320tetO | 320 repeats of tetO sponge |
| 160831 | pXWSPtet2 | 1 repeat of Ptet2 sponge |
| 160832 | pXWS5Ptet2 | 5 repeats of Ptet2 sponge |
| 160833 | pXWS10Ptet2 | 10 repeats of Ptet2 sponge |
| 160834 | pXWS20Ptet2 | 20 repeats of Ptet2 sponge |
| 160835 | pXWS40Ptet2 | 40 repeats of Ptet2 sponge |
| 160836 | pXWSLBS | 1 repeat of LBS sponge |
| 160837 | pXWS10LBS | 10 repeats of LBS sponge |
| 160838 | pXWS20LBS | 20 repeats of LBS sponge |
| 160839 | pXWS40LBS | 40 repeats of LBS sponge |
| 160840 | pXWS80LBS | 80 repeats of LBS sponge |
| 160841 | pXWSPecf11 | 1 repeat of Pecf11 sponge |
| 160842 | pXWS10Pecf11 | 10 repeats of Pecf11 sponge |
| 160843 | pXWS20Pecf11 | 20 repeats of Pecf11 sponge |
| 160844 | pXWS40Pecf11 | 40 repeats of Pecf11 sponge |
| 160845 | PXWS80LBS40tetO | LBS-tetO hybrid sponge with 80 repeats of LBS and 40 repeats of tetO |
| 160846 | PXWS80LBS320tetO | LBS-tetO hybrid sponge with 80 repeats of LBS and 320 repeats of tetO |
| 160847 | PXWS20LBS20Pecf11 | LBS-tetO hybrid sponge with 20 repeats of LBS and 20 repeat of Pecf11 |
| 160848 | PXWS80LBS40Pecf11 | LBS-tetO hybrid sponge with 80 repeats of LBS and 40 repeat of Pecf11 |