pXW101Plux2-33Ptet2-gfp
(Plasmid
#160820)
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PurposeTwo-layered AHL-responsive circuit containing a TetR-based inverter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSEVA121
- Backbone size w/o insert (bp) 3922
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePJ23101-30luxR-t-Plux2-33tetR-Ptet2-30gfp-t
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SpeciesSynthetic
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Insert Size (bp)2786
- Promoter PJ23101, Plux2, Ptet2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer AGGGCGGCGGATTTGTCC
- 3′ sequencing primer GCGGCAACCGAGCGTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXW101Plux2-33Ptet2-gfp was a gift from Baojun Wang (Addgene plasmid # 160820 ; http://n2t.net/addgene:160820 ; RRID:Addgene_160820) -
For your References section:
Synthetic protein-binding DNA sponge as a tool to tune gene expression and mitigate protein toxicity. Wan X, Pinto F, Yu L, Wang B. Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9. 10.1038/s41467-020-19552-9 PubMed 33235249
