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Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment.
Natwick DE, Collins SR
ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 10.1021/acssynbio.0c00511. Epub 2021 Apr 12. PubMed Article

Plasmids from Article

ID Plasmid Purpose
160999pLV_mTurquoise2-iLID-CAAXMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS
161000pLV_Lyn11-mTurquoise2-iLIDMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN
161001pLV_Stargazin-mTurquoise2-iLIDMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion
161002pLV_ADRB2-mTurquoise2-iLIDMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Antibodies from Article