An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice.
Zhang JF, Liu B, Hong I, Mo A, Roth RH, Tenner B, Lin W, Zhang JZ, Molina RS, Drobizhev M, Hughes TE, Tian L, Huganir RL, Mehta S, Zhang J
Nat Chem Biol. 2020 Sep 28. pii: 10.1038/s41589-020-00660-y. doi: 10.1038/s41589-020-00660-y. PubMed Article
Zhang JF, Liu B, Hong I, Mo A, Roth RH, Tenner B, Lin W, Zhang JZ, Molina RS, Drobizhev M, Hughes TE, Tian L, Huganir RL, Mehta S, Zhang J
Nat Chem Biol. 2020 Sep 28. pii: 10.1038/s41589-020-00660-y. doi: 10.1038/s41589-020-00660-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161753 | pcDNA3.1(+)-ExRai-AKAR2 | Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo. |
| 161754 | pcDNA3.1(+)-ExRai-AKAR2(T/A) | Negative-control mutant for ExRai-AKAR2 biosensor. |
| 161755 | pcDNA3-pmExRai-AKAR2 | Plasma membrane-targeted ExRai-AKAR2. |
| 161756 | pcDNA3-erExRai-AKAR2 | ExRai-AKAR2 targeted to the cytosolic face of the ER membrane. |
| 161757 | pcDNA3-mitoExRai-AKAR2 | ExRai-AKAR2 targeted to the outer mitochondrial membrane. |