SDR enzymes oxidize specific lipidic alkynylcarbinols into cytotoxic protein-reactive species.
Demange P, Joly E, Marcoux J, Zanon PRA, Listunov D, Rulliere P, Barthes C, Noirot C, Izquierdo JB, Rozie A, Pradines K, Hee R, de Brito MV, Marcellin M, Serre RF, Bouchez O, Burlet-Schiltz O, Oliveira MCF, Ballereau S, Bernardes-Genisson V, Maraval V, Calsou P, Hacker SM, Genisson Y, Chauvin R, Britton S
Elife. 2022 May 10;11. pii: 73913. doi: 10.7554/eLife.73913. PubMed Article
Demange P, Joly E, Marcoux J, Zanon PRA, Listunov D, Rulliere P, Barthes C, Noirot C, Izquierdo JB, Rozie A, Pradines K, Hee R, de Brito MV, Marcellin M, Serre RF, Bouchez O, Burlet-Schiltz O, Oliveira MCF, Ballereau S, Bernardes-Genisson V, Maraval V, Calsou P, Hacker SM, Genisson Y, Chauvin R, Britton S
Elife. 2022 May 10;11. pii: 73913. doi: 10.7554/eLife.73913. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161903 | pEGFP-N1-HSD17B11-WT-FLAGC | Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance. |
| 161904 | pEGFP-N1-HSD17B11-S172L-FLAGC | Expresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance. |
| 161915 | pEGFP-N1-HPGD-FLAGC | Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance. |
| 161916 | pE-N1-RDH11 | Expresses untagged human RDH11. Confers G418 resistance. |
| 161917 | pICE-EGFP-FLAG-PSMD2 | Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells. |
| 161918 | pICE-EGFP-FLAG-PLIN3 | Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells. |
| 161919 | pICE-EGFP-FLAG-TK1 | Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells. |
| 161920 | pEGFP-C1-FLAG-BRAT1 | Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance. |
| 161923 | pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11 | To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1. |
| 161924 | pCAG-eSpCas9-2A-GFP-sgRNA-RDH11 | To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2. |
| 184499 | pEGFP-N1-HSD17B11-L14P-FLAGC | Expresses the L14P mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance. |
| 184500 | pEGFP-N1-HSD17B11-V16D-FLAGC | Expresses the V16D mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance. |
| 184501 | pEGFP-N1-HSD17B13-WT-FLAGC | Expresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance. |