Rational design and implementation of a chemically inducible heterotrimerization system.
Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, Umehara T, Inoue T
Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x. Epub 2020 Aug 3. PubMed Article
Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, Umehara T, Inoue T
Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x. Epub 2020 Aug 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162425 | Lyn-YFP-FRB1C | for use in CIT (chemically induced trimerization) system |
| 162426 | Lyn-YFP-FRB2C | for use in CIT (chemically induced trimerization) system |
| 162427 | Lyn-YFP-FRB3C | for use in CIT (chemically induced trimerization) system |
| 162428 | FRB1N-CFP-CAAX | for use in CIT (chemically induced trimerization) system |
| 162429 | FRB2N-CFP-CAAX | for use in CIT (chemically induced trimerization) system |
| 162430 | FRB3N-CFP-CAAX | for use in CIT (chemically induced trimerization) system |
| 162431 | Lyn-YFP-FRB1N | for use in CIT (chemically induced trimerization) system |
| 162432 | mCh-FRB1C-Cb5 | for use in CIT (chemically induced trimerization) system |
| 162433 | CFP-FKBP | for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system |
| 162434 | Lyn-CFP-FRB1C_no-linker | for use in CIT (chemically induced trimerization) system |
| 162435 | YFP-FRB1N-Cb5 | for use in CIT (chemically induced trimerization) system |
| 162436 | Tom20-mCh-FKBP | for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system |
| 162437 | Lyn-YFP-FKBP1N | for use in CIT (chemically induced trimerization) system |
| 162438 | FKBP1C-CFP-Cb5 | for use in CIT (chemically induced trimerization) system |
| 162439 | Lyn-YFP-Clover-FRB1N | for use in CIT (chemically induced trimerization) system |
| 162440 | mRuby-PH-PLCdelta | for use in CIT (chemically induced trimerization) system |
| 162441 | iRFP-FRB1C-Cb5 | for use in CIT (chemically induced trimerization) system |