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Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes.
Sreekanth V, Zhou Q, Kokkonda P, Bermudez-Cabrera HC, Lim D, Law BK, Holmes BR, Chaudhary SK, Pergu R, Leger BS, Walker JA, Gifford DK, Sherwood RI, Choudhary A
ACS Cent Sci. 2020 Dec 23;6(12):2228-2237. doi: 10.1021/acscentsci.0c00129. Epub 2020 Nov 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
162723pX330 NL-FKBP-Cas9Mammalian expression
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162724pX330 LC-FKBP-Cas9Mammalian expression
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162725pX330 NC-FKBP-Cas9Mammalian expression
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162726pX330 NLC-FKBP-Cas9Mammalian expression
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162727pX330 N-FKBP-Cas9Mammalian expression
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162728pX330 L-FKBP-Cas9Mammalian expression
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162729pX330 C-FKBP-Cas9Mammalian expression
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162730pET SUMO NL-FKBP-Cas9Bacterial expression, protein expression
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162735pU6 SgRNA RBM20Mammalian expression
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162736pU6 SgRNA GAPDHMammalian expression
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Antibodies from Article