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Genome-wide CRISPR screens of oral squamous cell carcinoma reveal fitness genes in the Hippo pathway.
Chai AWY, Yee PS, Price S, Yee SM, Lee HM, Tiong VK, Goncalves E, Behan FM, Bateson J, Gilbert J, Tan AC, McDermott U, Garnett MJ, Cheong SC
Elife. 2020 Sep 29;9. pii: 57761. doi: 10.7554/eLife.57761.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
163138pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-WLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag
163147pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-WLentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag
163169pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-WLentiviral plasmid with non-targeting gRNA, co-expression of BFP tag
163170pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-WLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag
163172pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-WLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag
163174pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-WLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag
163175pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-WLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag
163176pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-WLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag
163177pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-WLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag
163178pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-WLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag
163179pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-WLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Antibodies from Article