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NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins.
Scheu AHA, Lim SYT, Metzner FJ, Mohammed S, Howarth M
Nat Commun. 2021 Jan 29;12(1):717. doi: 10.1038/s41467-021-20963-5. PubMed Article

Plasmids from Article

ID Plasmid Purpose
163613pET28a-His6-OAZ-GSY-SPM-CtagExpresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.
163614pET28a-His6-ODC-CtagExpresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.
163615pET28a-TGFα-GSY-SPMExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

Antibodies from Article