PurposeExpresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||163614||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5246
- Total vector size (bp) 6689
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1443
Entrez GeneODC1 (a.k.a. NEDBA, NEDBIA, ODC)
- Promoter T7
/ Fusion Proteins
- His6 (N terminal on backbone)
- C-tag (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-His6-ODC-Ctag was a gift from Mark Howarth (Addgene plasmid # 163614 ; http://n2t.net/addgene:163614 ; RRID:Addgene_163614)
For your References section:NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Scheu AHA, Lim SYT, Metzner FJ, Mohammed S, Howarth M.. Nat Commun 12, 717 (2021). 10.1038/s41467-021-20963-5