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A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre C, de Los Santos EL, Freemont PS
ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
163756pTU1-A-SP44-mScarlet-IExpresses Streptomyces codon-optimised mScarlet-I
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163757pTU1-A-SP44-sfGFPExpresses Streptomyces codon-optimised sfGFP
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163763pTU1-A-SP44-mVenus-IExpresses Streptomyces codon-optimised mVenus-I
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172430pTU1-B-SP44s-GUSExpresses Streptomyces codon-optimised beta-D-glucuronidase
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172431pSF1C-A-RFPStrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)
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172432pSF2C-A-RFPStrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)
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