pTU1-B-SP44s-GUS
(Plasmid
#172430)
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PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidase
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTU1-B
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Backbone manufacturerFreemont lab
- Backbone size w/o insert (bp) 2296
- Total vector size (bp) 4105
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-D-glucuronidase
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SpeciesEscherichia coli
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Insert Size (bp)1809
- Promoter SP44s
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer VF2
- 3′ sequencing primer VR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.16.384693v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTU1-B-SP44s-GUS was a gift from Paul Freemont & Simon Moore (Addgene plasmid # 172430 ; http://n2t.net/addgene:172430 ; RRID:Addgene_172430) -
For your References section:
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology. Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre C, de Los Santos EL, Freemont PS. ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 10.1021/acssynbio.0c00581. Epub 2021 Jan 26. 10.1021/acssynbio.0c00581 PubMed 33497199
Map uploaded by the depositor.
