Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons.
Petassi MT, Hsieh SC, Peters JE
Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 10.1016/j.cell.2020.11.005. PubMed Article
Petassi MT, Hsieh SC, Peters JE
Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 10.1016/j.cell.2020.11.005. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164260 | pMTP112 (pMS26_miniTn6900(KanR)) | Delivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element. |
| 164261 | pMTP130 (pTA106_TnsABC(Tn6900)) | Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter. |
| 164262 | pMTP140 (pBAD322G_TniQ-Cascade(Tn6900)) | Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter. |
| 164263 | pMTP170 (pBAD33_CRISPR(Tn6900)-entry) | Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array. |