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Addgene

pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
(Plasmid #164262)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164262 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD322G
  • Backbone size w/o insert (bp) 4357
  • Total vector size (bp) 9320
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
  • Species
    Aeromonas salmonicida
  • Insert Size (bp)
    5006
  • GenBank ID
    NZ_CP022176.1 DQ119284.1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for vector backbone.
Cronan, J. E. (2006). A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control. Plasmid, 55(2), 152–157. http://doi.org/10.1016/j.plasmid.2005.07.001

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTP140 (pBAD322G_TniQ-Cascade(Tn6900)) was a gift from Joseph Peters (Addgene plasmid # 164262 ; http://n2t.net/addgene:164262 ; RRID:Addgene_164262)
  • For your References section:

    Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons. Petassi MT, Hsieh SC, Peters JE. Cell. 2020 Nov 30. pii: S0092-8674(20)31464-1. doi: 10.1016/j.cell.2020.11.005. 10.1016/j.cell.2020.11.005 PubMed 33271061