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Screening for functional circular RNAs using the CRISPR-Cas13 system.
Li S, Li X, Xue W, Zhang L, Yang LZ, Cao SM, Lei YN, Liu CX, Guo SK, Shan L, Wu M, Tao X, Zhang JL, Gao X, Zhang J, Wei J, Li J, Yang L, Chen LL
Nat Methods. 2021 Jan;18(1):51-59. doi: 10.1038/s41592-020-01011-4. Epub 2020 Dec 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
165070p23-NES-LwaCas13a-msfGFP-NES-Flagoverexpression of LwaCas13a in human cells
165071p23-NES-PspCas13b-msfGFP-NES-Flagoverexpression of PspCas13b in human cells
165072p23-NES-PguCas13b-msfGFP-NES-Flagoverexpression of PguCas13b in human cells
165073p23-NES-RanCas13b-msfGFP-NES-Flagoverexpression of RanCas13b in human cells
165074p23-NES-EsCas13d-msfGFP-NES-Flagoverexpression of EsCas13d in human cells
165075p23-NES-AdmCas13d-msfGFP-NES-Flagoverexpression of AdmCas13d in human cells
165076p23-NES-RfxCas13d-msfGFP-NES-Flagoverexpression of RfxCas13d in human cells
165077p23-NLS-RfxCas13d-mcherry-NLS-Flagoverexpression of RfxCas13d in human cells
165078RfxCas13d-backboneExpression of RfxCas13d sgRNAs from a U6 promoter

Antibodies from Article