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Cas9 deactivation with photocleavable guide RNAs.
Zou RS, Liu Y, Wu B, Ha T
Mol Cell. 2021 Apr 1;81(7):1553-1565.e8. doi: 10.1016/j.molcel.2021.02.007. Epub 2021 Mar 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
165157pET42b-AncBE4maxExpresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression
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165158pLPC-mCh-ACTB-P2A-EGFPExpresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells
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