David Andrews Lab:
Andrew lab Venus and Cerulean fusions
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166762 | Venus-30aa-Noxa-pEGFP-C1 | To increase the length of the linker between Venus and Noxa proteins |
| 166763 | Venus-79aa-ActA-pEGFP-C1 | To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments. |
| 166764 | Venus-7aa-Cb5-pEGFP-C1 | To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments. |
| 166765 | Venus-7aa-MAO-pEGFP-C3 | To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments. |
| 167529 | Venus-30aa-Noxa-4E-pEGFP-C1 | To increase the length of the linker between Venus and Noxa-4E proteins |