Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity.
Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, Minasov G, Satchell KJF, Dong M, Liu DR
Science. 2021 Feb 19;371(6531):803-810. doi: 10.1126/science.abf5972. PubMed Article
Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, Minasov G, Satchell KJF, Dong M, Liu DR
Science. 2021 Feb 19;371(6531):803-810. doi: 10.1126/science.abf5972. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167995 | AP-SNAP25 | positive selection PACE Accessory Plasmid |
| 167996 | AP-VAMP1 | positive selection PACE Accessory Plasmid |
| 167997 | AP-Ykt6 | positive selection PACE Accessory Plasmid |
| 167998 | AP-VAMP7(low) | positive selection PACE Accessory Plasmid |
| 167999 | AP-VAMP1(X) | positive selection PACE Accessory Plasmid |
| 168000 | AP-VAMP4 | positive selection PACE Accessory Plasmid |
| 168001 | AP-VAMP5 | positive selection PACE Accessory Plasmid |
| 168002 | APT3-VAMP7 | positive selection PACE Accessory Plasmid |
| 168003 | APT3-Ykt6 | positive selection PACE Accessory Plasmid |
| 168004 | APT3-VAMP4 | positive selection PACE Accessory Plasmid |
| 168005 | AP-PTEN(med) | positive selection PACE Accessory Plasmid |
| 168006 | APT3-PTEN (PTEN high) | positive selection PACE Accessory Plasmid |
| 168007 | APneg-VAMP1a | negative selection PACE Accessory Plasmid |
| 168008 | APneg-VAMP1b | negative selection PACE Accessory Plasmid |
| 168009 | APneg-VAMP1c | negative selection PACE Accessory Plasmid |
| 168010 | APneg-VAMP1d | negative selection PACE Accessory Plasmid |
| 168011 | APneg-SNAP25a | negative selection PACE Accessory Plasmid |
| 168012 | APneg-SNAP25b | negative selection PACE Accessory Plasmid |
| 168013 | APneg-SNAP25c | negative selection PACE Accessory Plasmid |
| 168014 | APneg-SNAP25d | negative selection PACE Accessory Plasmid |
| 168015 | pET-mBoNT/F | Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells |
| 168016 | pET-mF(3230)A3 | Expresses MBP-F(3230)A3-6xHis in bacterial cells |
| 168017 | pET-BoNT/X | Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells |
| 168018 | pET-X(4130)B1 | Expresses X(4130)B1-6xHis in bacterial cells |
| 168019 | pET-X(5010)B1 | Expresses X(5010)B1-6xHis in bacterial cells |
| 168020 | pET-BoNT/E | Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells |
| 168021 | pET-E(4130)A2 | Expresses E(4130)A2-6xHis in bacterial cells |
| 168022 | pET-CV-VAMP1 | Expresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cells |
| 168023 | pET-CV-VAMP2 | Expresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cells |
| 168024 | pET-CV-VAMP3 | Expresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cells |
| 168025 | pET-CV-VAMP4 | Expresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cells |
| 168026 | pET-CV-VAMP5 | Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells |
| 168027 | pET-CV-VAMP7 | Expresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cells |
| 168028 | pET-CVm-VAMP8 | Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells |
| 168029 | pET-CVm-Sec22b | Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells |
| 168030 | pET-CV-Ykt6 | Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells |
| 168031 | pET-CV-SNAP25 | Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells |
| 168032 | pET-CVm-PTEN | Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells |