APT3-PTEN (PTEN high)
(Plasmid
#168006)
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Purposepositive selection PACE Accessory Plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneSC101
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Backbone manufacturerDavid Liu Lab
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePTEN-T3'PAP
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional insert: gIII/luxAB
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
APT3-PTEN (PTEN high) was a gift from David Liu (Addgene plasmid # 168006 ; http://n2t.net/addgene:168006 ; RRID:Addgene_168006) -
For your References section:
Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity. Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, Minasov G, Satchell KJF, Dong M, Liu DR. Science. 2021 Feb 19;371(6531):803-810. doi: 10.1126/science.abf5972. 10.1126/science.abf5972 PubMed 33602850
