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Genetically Encoded CRISPR Components Yield Efficient Gene Editing in the Invasive Pest Drosophila suzukii.
Kandul NP, Belikoff EJ, Liu J, Buchman A, Li F, Yamamoto A, Yang T, Shriner I, Scott MJ, Akbari OS
CRISPR J. 2021 Oct;4(5):739-751. doi: 10.1089/crispr.2021.0032. PubMed Article

Plasmids from Article

ID Plasmid Purpose
169010pB[U6-3 wex2 PUb-DsRed]For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.
169011pB[nos-Cas9 PUb-ZsGreen]For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.
169012pB[vasa-Cas9 PUb-ZsGreen]For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Antibodies from Article