Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp12/7/8 RNA-dependent RNA polymerase.
Bertolin AP, Weissmann F, Zeng J, Posse V, Milligan JC, Canal B, Ulferts R, Wu M, Drury LS, Howell M, Beale R, Diffley JFX
Biochem J. 2021 Jul 16;478(13):2425-2443. doi: 10.1042/BCJ20210200. PubMed Article
Bertolin AP, Weissmann F, Zeng J, Posse V, Milligan JC, Canal B, Ulferts R, Wu M, Drury LS, Howell M, Beale R, Diffley JFX
Biochem J. 2021 Jul 16;478(13):2425-2443. doi: 10.1042/BCJ20210200. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169178 | pBIG1b_nsp7 (SARS-CoV-2) | Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells |
| 169179 | pBIG1c_nsp8 (SARS-CoV-2) | Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells |
| 169180 | pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2) | Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells |
| 169181 | pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2) | Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells |
| 169182 | pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2) | Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells |
| 169183 | pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2) | Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells |
| 169184 | pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2) | Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells |
| 169185 | pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2) | Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells |
| 169186 | pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2) | To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli |
| 169187 | pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2) | To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli |
| 169188 | pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2) | To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli |