Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A.
Cook TB, Jacobson TB, Venkataraman MV, Hofstetter H, Amador-Noguez D, Thomas MG, Pfleger BF
Metab Eng. 2021 Jun 24;67:112-124. doi: 10.1016/j.ymben.2021.06.004. PubMed Article
Cook TB, Jacobson TB, Venkataraman MV, Hofstetter H, Amador-Noguez D, Thomas MG, Pfleger BF
Metab Eng. 2021 Jun 24;67:112-124. doi: 10.1016/j.ymben.2021.06.004. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169240 | pCas9-beta | Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering |
| 169241 | pCas9-beta-mmr | Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations |
| 169243 | pNVLTv2-pvdIJD-Ptrc-base | Suicide vector for integrating expression cassette in P. putida (IPTG induction) |
| 169244 | pNVLTv2-pvdIJD-Prha-base | Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction) |
| 169245 | pNVLTv2 | Generic suicide vector for knockouts and integrations |
| 169872 | pNVL-pvdIJD | Integration vector for deleting pvdIJD locus in P. putida |
| 169873 | pgRNAkan-glpR | Single guide RNA plasmid for Cas9 targeting glpR in P. putida |
| 169874 | pgRNAtet-phaZ | Single guide RNA plasmid for Cas9 targeting phaZ in P. putida |