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Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment.
Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H
Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 10.1016/j.immuni.2021.04.024. Epub 2021 May 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
169948pcDNA3.1 LIC 6A CARD8-FLAGMammalian expression of CARD8-FLAG
169949pcDNA3.1 LIC 6A CARD8-FLAG S297AMammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)
169950pcDNA3.1 LIC 6A CARD8-FLAG E274RMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)
169951pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297AMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)
169952pcDNA3.1 LIC 6A CARD8-FLAG L368GMammalian expression of CARD8-FLAG with a site III mutation (L368G)
169953pcDNA3.1 LIC 6A CARD8-FLAG F370GMammalian expression of CARD8-FLAG with a site III mutation (F370G)
169954pcDNA3.1 LIC 6A CARD8-FLAG R394EMammalian expression of CARD8-FLAG with a site III mutation (R394E)
169955pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297AMammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)
169956pcDNA3.1 LIC 6A CARD8-FLAG F405GMammalian expression of CARD8-FLAG with a site III mutation (F405G)
169957pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297AMammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)
169958pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAGMammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)
169959pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368GMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)
169960pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370GMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)
169961pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394EMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)
169962pDONR221 CARD8pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)
169963pDONR221 CARD8 FIINDpDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)
169964pDONR221 CARD8 ZUCpDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)
169965pDONR221 CARD8 Ub-S297pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)
169966pDONR221 CARD8 FIIND S297ApDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)
169967pDONR221 CARD8 FIIND E274RpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)
169968pDONR221 CARD8 FIIND E274R + S297ApDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)
169969pDONR221 CARD8 FIIND L368GpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)
169970pDONR221 CARD8 FIIND L370GpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)
169971pDONR221 CARD8 FIIND R394EpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)
169972pDONR221 CARD8 FIIND R394E + S297ApDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)
169973pDONR221 CARD8 FIIND F405GpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)
169974pDONR221 CARD8 FIIND F405G + S297ApDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)
169975pDONR221 CARD8 PAMpDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations
169976pDONR221 CARD8 PAM S297ApDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations
169977pDONR221 CARD8 PAM E274RpDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations
169978pDONR221 CARD8 PAM L368GpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations
169979pDONR221 CARD8 PAM F370GpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations
169980pDONR221 CARD8 PAM R394EpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations
169981pDONR221 CARD8 PAM F405GpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations
169982pInducer20 CARD8-HA PAMTet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations
169983pInducer20 CARD8-HA PAM S297ATet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations
169984pInducer20 CARD8-HA PAM E274RTet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations
169985pInducer20 CARD8-HA PAM L368GTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations
169986pInducer20 CARD8-HA PAM F370GTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations
169987pInducer20 CARD8-HA PAM R394ETet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations
169988pInducer20 CARD8-HA PAM F405GTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations
169989pLEX305-N-dTAG-CARD8Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)
169990pLEX305-N-dTAG-CARD8-ZUCMammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).
169991pcDNA3.1 CARD8-TEV-GFP-FLAGMammalian purification construct for CARD8
169992pcDNA3.1 CARD8-TEV-GFP-FLAG S297AMammalian purification construct for CARD8 with an autoprocessing-deficient mutation
170119pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405GMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

Antibodies from Article