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Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment.
Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H
Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
169948pcDNA3.1 LIC 6A CARD8-FLAGMammalian expression of CARD8-FLAG
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169949pcDNA3.1 LIC 6A CARD8-FLAG S297AMammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)
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169950pcDNA3.1 LIC 6A CARD8-FLAG E274RMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)
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169951pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297AMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)
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169952pcDNA3.1 LIC 6A CARD8-FLAG L368GMammalian expression of CARD8-FLAG with a site III mutation (L368G)
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169953pcDNA3.1 LIC 6A CARD8-FLAG F370GMammalian expression of CARD8-FLAG with a site III mutation (F370G)
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169954pcDNA3.1 LIC 6A CARD8-FLAG R394EMammalian expression of CARD8-FLAG with a site III mutation (R394E)
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169955pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297AMammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)
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169956pcDNA3.1 LIC 6A CARD8-FLAG F405GMammalian expression of CARD8-FLAG with a site III mutation (F405G)
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169957pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297AMammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)
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169958pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAGMammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)
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169959pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368GMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)
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169960pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370GMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)
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169961pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394EMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)
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169962pDONR221 CARD8pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)
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169963pDONR221 CARD8 FIINDpDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)
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169964pDONR221 CARD8 ZUCpDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)
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169965pDONR221 CARD8 Ub-S297pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)
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169966pDONR221 CARD8 FIIND S297ApDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)
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169967pDONR221 CARD8 FIIND E274RpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)
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169968pDONR221 CARD8 FIIND E274R + S297ApDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)
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169969pDONR221 CARD8 FIIND L368GpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)
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169970pDONR221 CARD8 FIIND L370GpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)
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169971pDONR221 CARD8 FIIND R394EpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)
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169972pDONR221 CARD8 FIIND R394E + S297ApDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)
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169973pDONR221 CARD8 FIIND F405GpDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)
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169974pDONR221 CARD8 FIIND F405G + S297ApDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)
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169975pDONR221 CARD8 PAMpDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations
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169976pDONR221 CARD8 PAM S297ApDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations
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169977pDONR221 CARD8 PAM E274RpDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations
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169978pDONR221 CARD8 PAM L368GpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations
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169979pDONR221 CARD8 PAM F370GpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations
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169980pDONR221 CARD8 PAM R394EpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations
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169981pDONR221 CARD8 PAM F405GpDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations
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169982pInducer20 CARD8-HA PAMTet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations
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169983pInducer20 CARD8-HA PAM S297ATet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations
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169984pInducer20 CARD8-HA PAM E274RTet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations
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169985pInducer20 CARD8-HA PAM L368GTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations
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169986pInducer20 CARD8-HA PAM F370GTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations
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169987pInducer20 CARD8-HA PAM R394ETet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations
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169988pInducer20 CARD8-HA PAM F405GTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations
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169989pLEX305-N-dTAG-CARD8Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)
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169990pLEX305-N-dTAG-CARD8-ZUCMammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).
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169991pcDNA3.1 CARD8-TEV-GFP-FLAGMammalian purification construct for CARD8
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169992pcDNA3.1 CARD8-TEV-GFP-FLAG S297AMammalian purification construct for CARD8 with an autoprocessing-deficient mutation
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170119pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405GMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).
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Recombinant Antibodies from Article