pcDNA3.1 LIC 6A CARD8-FLAG
PurposeMammalian expression of CARD8-FLAG
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||169948||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 LIC 6A
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCARD8 (a.k.a. CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV F
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 LIC 6A CARD8-FLAG was a gift from Hao Wu (Addgene plasmid # 169948 ; http://n2t.net/addgene:169948 ; RRID:Addgene_169948)
For your References section:Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H. Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 10.1016/j.immuni.2021.04.024. Epub 2021 May 20. 10.1016/j.immuni.2021.04.024 PubMed 34019797